Bioprocess Engineering towards green chemicals
In order to reduce CO2 emmissions and the use of fossil resources, the microbial production platform offers a vast range of possibilities. Many different compounds and/or precursors can be produced by mircoorganisms and their productivity can be enhanced by means of genetic modifications.
Within this project, we aim to develop a bioporcess for the efficient production of a volatile chemical compound. Three pathways are being designed and the resulting strains are tested and evaluated in their performance of producing the compound of interest.
Further aspects to consider during process development are product inhibition and in-situ product removal. Here, a mathematical model can help to make predictions and to identify and optimize significant parameters of the process. This enables more reliable comparison of the different approaches and pathways.
Starting from aerobic production, two pathway engineering approaches will be pursued that should improve the anaerobic production of the compound of interest. Both anaerobic pathways aim at improving the product yield on substrate. However, their competitiveness towards the aerobic process in terms of productivity and overall costs still needs to be evaluated. The present case, thus contributes to the latest discussion on whether aerobic or anaerobic processes should be pursued for microbial production of (bulk) chemicals in the future.