My research focuses on developing a generic tool to improve plant regeneration processes in a germplasm-independent manner. Embryo-expressed transcription factors like the AP2 domain protein BABY BOOM and the CAAT-box binding factor LEAFY COTYLEDON1 have been used to enhance plant regeneration in a range of crops when stably expressed from a constitutive promoter. We aim to overcome this problem by transiently activating endogenous BBM/LEC1 gene expression using CRISPR-dCas9 technology. The ability to use different activators fused to a dCas9 protein to regulate LEC1/BBM gene expression will be evaluated. Additionally we aim to identify transcriptional activator or repressor binding sites in the arabidopsis BBM/LEC1 promoters by producing deletions of various sizes via CRISPR/Cas9.